Answer

No. A Q-SYS Core will not be able to connect to a peripheral if they are not both talking over the same Lan NIC. That is, Lan A of a Core cannot talk to Lan B of a peripheral or vice versa. If you attempt to connect them in this way, the peripheral(s) will appear as "missing" in the inventory.

Note: Q-SYS peripherals need to be run over Lan A. The only scenario where a Core would connect to peripherals over Lan B would be in a network redundant situation where the Lan A network has failed.